Irving Boime, Ph.D.
Departments of Developmental Biology and Obstetrics and Gynecology
The placental hormone human chorionic gonadotropin (CG), and the pituitary hormones lutropin (LH), follitropin (FSH), and thyrotropin (TSH) are a family of heterodimeric glycoproteins that share a common alpha subunit but differ in their hormone-specific subunits. One or more of these hormones is essential for gonadal development and maintaining pregnancy and thyroid function. Our laboratory is focused in the following general area:
Structure-function studies. Using site-directed mutagenesis, we are studying the structural determinants that govern the unique post- translational modifications of the related placental and pituitary glycoprotein hormones. These hormones undergo specific modifications and have unique biologic activities. Together with DNA mediated transfection techniques, monoclonal antibody screening, and protein and carbohydrate characterization, we can determine: (a) the region in the hormone associated with unique biologic activity; (b) the region critical for subunit assembly; and (c) disulfide bonds for folding of these hormones and the sequences critical for the differential sorting of the pituitary hormones. Studies are also underway for determining the ligand determinants which are critical for signal transduction in vivo as well as dissecting the structure of the gonadal receptors associated with the biological action of these hormones. Such information has been useful for designing clinically useful agents for treating infertility. A major focus of the laboratory is elucidating the epitopes that govern the differential sorting of these hormones in unique pituitary secretion pathways.
- Pearl, C., Jablonka-Shariff, A., and Boime, I. “Re-routing of an FSH analog to the regulated secretory pathway.” Endocrinology." 151, 388-93 (2010).
- Pearl, C ,Boime, I. “Sulfation of LH does not affect intracellular trafficking.” Mol & Cell Endo. 309, 76-81 (2009).
- Jennings, M.W., Boime*, I., Daphna-Iken, D., Jablonka-Shariff, A., Conley, A.J., Colgin, M., Bidstrup, L.A., Meyers-Brown, G. A., Famula, T.R., Roser, J.F*. “The efficacy of recombinant equine follicle stimulating hormone (reFSH) to promote follicular growth in mares using a follicular suppression model.” Anim. Reprod. Sci. (2009). *Both laboratories contributed equally to this work.
- Fauser, B.C., Mannaerts, B.M., Devroey, P., Leader, A., Boime, I. and Baird, D.T. “Advances in recombinant DNA technology: corifollitropin alfa, a hybrid molecule with sustained follicle-stimulating activity and reduced injection frequency.” Hum. Reprod. Update. (2009).
- Jablonka-Shariff, A., Boime, I. “Secretory trafficking signal encoded in the carboxyl-terminal region of the CGβ subunit.” Molec. Endocrinol. (Baltimore, Md, 23, 316-23 (2009).
- Adams, T. E. and I. Boime. "The expanding role of recombinant gonadotropins in assisted reproduction." Reprod Domest Anim 43 Suppl 2: 186-92 (2008).
- Jablonka-Shariff, A., Pearl, C.A., Comstock, A., Boime, I. "A Carboxyl-terminal Sequence in the Lutropin β subunit contributes to the sorting of Lutropin to the Regulated Pathway." J. Biol. Chem, 283(17): 11485-11492 (2008).
- Lemke, E.P., Adams, B.M., Jablonka-Shariff, A., Boime, I., Adams, T.E. “Single-chain human gonadotropin analogs induce follicle development in sheep.” J. Endocrinol, 196, 593-600 (2008).
- 166. Yoon, M.J., Boime, I., Colgin, M., Niswender, K.D., King, S.S., Alvarenga, M., Jablonka-Shariff, A., Pearl, C. and Roser, J.F. “The efficacy of a single chain recombinant equine luteinizing hormone (reLH): Induction of ovulation, hormone profiles, and inter-ovulatory intervals.” Domestic Animal Endocrinol, 33, 470-9 (2007).
- 165. Antenos, M., Stemler, M., Boime, I., and Woodruff, T.K. “N-linked oligosaccharides direct the differential assembly and secretion of inhibin α- and βA-subunit dimers.” Molec. Endocrinol., 21, 1670-84 (2007).
- 164. Jablonka-Shariff, A., Roser, J.F., Bousfield, G.R., Wolfe, M.W., Sibley, L.E., Colgin, M., and Boime, I. “Expression and bioactivity of a single chain recombinant equine luteinizing hormone (reLH).” Theriogenology 67, 311-320 (2007).
For a complete list of Dr. Boime's publications, click here
- St. Louis College of Pharmacy, St. Louis, MO B.S. 1964 Pharmacology
- Purdue University, West Lafayette, IN M.S. 1966 Pharmacology
- Washington University, St. Louis, MO Ph.D. 1970 Pharmacology
- Professor, Departments of Pharmacology and Obstetrics/Gynecology, Washington University School of Medicine, 1981 - present
- Associate Professor, Departments of Pharmacology and Obstetrics/Gynecology, Washington University School of Medicine, 1977 - 1981
- Assistant Professor, Departments of Pharmacology and Obstetrics/Gynecology, Washington University School of Medicine, 1972 - 1977
- Postdoctoral Fellow of the American Cancer Society in the laboratory of Dr. Philip
- Fellow of the American Cancer Society, 1970 - 1972
- U.S. Public Health Service, Research Career Development Award, 1977 - 1982
- Population Research Committee of the NIH, 1991 - present
- Member of Biochemical Endocrinology Study Section, National Institutes of Health, 1983 - 1987
Editorial Board Memberships
- Endocrinology (Associate Editor), 1991 -1992
- Journal of Biological Chemistry, 1981 -1990 & 1993 - present
- Placenta, 1985 - present
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- Faculty Members
- Lilianna (Lila) Solnica-Krezel, Ph.D.
- Irving Boime, Ph.D.
- Angela Bowman, Ph.D.
- Aaron DiAntonio, M.D., Ph.D.
- Douglas F. Covey, Ph.D.
- Gregory A. Grant, Ph.D.
- Shin-ichiro Imai, M.D., Ph.D.
- S. Kerry Kornfeld, M.D., Ph.D
- Kristen Kroll, Ph.D.
- Craig Micchelli, Ph.D.
- Kelly Monk, Ph.D.
- Samantha A. Morris, Ph.D.
- Jeanne Nerbonne, Ph.D.
- David M. Ornitz, M.D., Ph.D.
- Zachary Pincus, Ph.D.
- Stacey Rentschler, M.D., Ph.D.
- John H. Russell, Ph.D.
- Andrew S. Yoo, Ph.D.
- Bo Zhang, Ph.D.
- Adjunct Faculty
- Joint Faculty Members
- Research Interests
- Emeritus Faculty
- Former Faculty
- Model Systems