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Washington University in St. Louis - School of Medicine
Department of Developmental Biology

Microscopy

Department of Developmental Biology Microscopy Core


The Department of Developmental Biology’s Microscopy Core has a Laser Microdissection Microscope available to the faculty in the department and across the university.  The microscope was purchased August 2010, and the laser unit has recently been replaced to a new one.


Overview:  Laser microdissection (LMD) is an automated sample preparation technique that enables isolation of specific cells from a mixed population under microscopic visualization. This technique of isolating a pure sample from a heterogeneous mixture allows for more efficient and accurate results with downstream microgenomics applications such as RNA sequencing, quantitative RT-PCR, and proteomics.


The LMD technique can be used to isolate just a few cells or even a single cell from tissue samples. LMD is precise and targeted. LMD allows one to obtain answers in a single cell that may otherwise have been missed in a whole tissue scrape that contains a heterogeneous mixture of healthy, stromal and cancer cells.


Equipment:   Leica Microdissection System (Leica LMD7000)


Component Parts include: Laser Capture Microscope with Hitachi HVD20 Camera, Laser CTR6500, Mercury Bulb, Control box for the Laser LMD7000, Computer, Touch Screen Monitor and Regular Monitor Screen


Services:
• Time on the Leica Microdissection System: $25/hour. 
• Training on the Leica Microdissection System: $25/hour


CONTACT: Cindy Brace (Imai lab); (314) 747-1031; c.brace@wustl.edu.  Anybody who would like to use this LMD system has to take a training session by Cindy before using the system.

 

To access the Microscopy core web site, click here.

Upcoming Events

Nov 26

Mayssa Mokalled, Ph.D.

Department of Developmental Biology Seminar Series

Assistant Professor Department of Developmental Biology Washington University School…

Nov 28

Jasmin Sponagel (Josh Rubin Lab)/ Shanshan Song (Aaron Johnson lab)

RAD Research Forum Series

12:00 pm, Needleman Library

Dec 3

William Mair, Ph.D.

Department of Developmental Biology Seminar Series

Associate Professor of Genetics and Complex Diseases Department of…

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