Department of Developmental Biology Microscopy Core
The Department of Developmental Biology’s Microscopy Core has a Laser Microdissection Microscope available to the faculty in the department and across the university. The microscope was purchased August 2010, and the laser unit has recently been replaced to a new one.
Overview: Laser microdissection (LMD) is an automated sample preparation technique that enables isolation of specific cells from a mixed population under microscopic visualization. This technique of isolating a pure sample from a heterogeneous mixture allows for more efficient and accurate results with downstream microgenomics applications such as RNA sequencing, quantitative RT-PCR, and proteomics.
The LMD technique can be used to isolate just a few cells or even a single cell from tissue samples. LMD is precise and targeted. LMD allows one to obtain answers in a single cell that may otherwise have been missed in a whole tissue scrape that contains a heterogeneous mixture of healthy, stromal and cancer cells.
Equipment: Leica Microdissection System (Leica LMD7000)
Component Parts include: Laser Capture Microscope with Hitachi HVD20 Camera, Laser CTR6500, Mercury Bulb, Control box for the Laser LMD7000, Computer, Touch Screen Monitor and Regular Monitor Screen
• Time on the Leica Microdissection System: $25/hour.
• Training on the Leica Microdissection System: $25/hour
CONTACT: Cindy Brace (Imai lab); (314) 747-1031; firstname.lastname@example.org. Anybody who would like to use this LMD system has to take a training session by Cindy before using the system.
To access the Microscopy core web site, click here.
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